Volume 3, Issue 1, March 2020, Page: 10-16
The Ultrastructure of Chrysemys picta Eggshell and Shell Membrane
Jessica Gibbons, The Department of Biological Sciences, State University New York Oswego, Oswego, USA
Suresh Nimmagadda, The Department of Biological Sciences, State University New York Oswego, Oswego, USA
Poongodi Geetha-Loganathan, The Department of Biological Sciences, State University New York Oswego, Oswego, USA
Received: Jan. 8, 2020;       Accepted: Jan. 20, 2020;       Published: Feb. 11, 2020
DOI: 10.11648/j.ajz.20200301.13      View  310      Downloads  152
Abstract
The structure of eggshells plays a multifunctional role in successful embryogenesis. The avian eggshells have been extensively studied while there are few studies on reptilian eggshells. Painted turtle eggs exhibit pliable shells and have received attention by researchers because of its availability but general morphological characteristics of their eggshells have not been previously reported. The objective of the study is to describe the ultrastructure of the eggshell and shell membranes of preincubated and post incubated eggs of painted turtles (Chrysemys picta) using a Scanning Electron Microscope (SEM). Painted turtles lay oval-shaped, pliable-shelled eggs, eggshell is composed of outer calcareous layer and inner shell membrane. Outer mineral layer composed of loosely arranged shell units separated with pores traversing through the calciferous layer to the membrane. The size of shell units and pores increase with incubation suggests that the developing embryo derives calcium and water from the eggshell. The outer mineral layer is connected to the multilayered shell membrane at the basal knob. The outer shell membrane is composed of randomly arranged fibers organized into rough, filamentous meshwork and the inner membrane is in contact with the albumin consist of smooth, parallel arranged, featureless f ibers. In conclusion, eggs of painted turtle showed some similarities in morphology to other species of turtles that lay pliable-shelled eggs, the description on structural characteristics of an eggshell is essential to understand the chemical composition of the eggshell and its development.
Keywords
Painted Turtle, Eggshell, Shell Membrane, Scanning Electron Microscope, Shell Units, Shell Membrane
To cite this article
Jessica Gibbons, Suresh Nimmagadda, Poongodi Geetha-Loganathan, The Ultrastructure of Chrysemys picta Eggshell and Shell Membrane, American Journal of Zoology. Vol. 3, No. 1, 2020, pp. 10-16. doi: 10.11648/j.ajz.20200301.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
A. S. Romer, “Origin of the Amniote Egg”, The Scientific Monthly, vol. 85, pp. 57-63, 1957.
[2]
H. H. Schleich H, W. Kastle, “Reptile egg-shells SEM atlas”, Gustav Fischer, Stuttgart, 1988.
[3]
T. G. Benton, S. J. Plaistow, A. P. Beckerman, C. T. Lapsley, S. Littlejohns, ‘Changes in maternal investment in eggs can affect population dynamics’, Proceedings of the Royal Society B: Biological Sciences, vol. 272, pp. 1351–1356, 2005.
[4]
M. J. Packard, G. C. Packard, T. J. Boardman, “Structure of eggshells and water relations of reptilian eggs”. Herpetologica, vol. 38, pp. 136-155, 1982.
[5]
G. Sahoo, B. K. Mohapatra, R. K. Sahoo, P. Mohanty-Hejmadi, “Ultrastructure and Characteristics of Eggshells of the Olive Ridley Turtle (Lepidochelys olivacea) from Gahirmatha, India”. Acta Anatomica. vol. 156, pp. 261-267, 1996.
[6]
M. J. Packard, “Ultrastructural morphology of the shell and shell membrane of eggs of common snapping turtles (Chelydra serpentina)”. J. Morphol, vol. 165, pp. 187-204, 1980.
[7]
G. C. Packard, T. L. Taigen, M. J. Packard, and R. D. Shu-man, “Water-vapor conductance of testudinian and crocodilian eggs (Class Reptilia)”. Respir. Phyeicl, vol. 38, pp. 1-10, 1979.
[8]
S. E. Solomon, and J. M. Watt, “The structure of the eggshell of the leatherback turtle (Dermochelys coriacea)”. Anim. Technol, vol. 36, pp. 19-27, 1985.
[9]
J. D. Miller, “Embryology of marine turtles”. Ph. D. Thesis, Univ. New England, Armidale, New South Wales. 1982.
[10]
M. J. Packard, J. B. Iverson and G. C. Packard, “Morphology of shell formation in eggs of the turtle Kinosternonflavescens”. J. Morphol, vol. 181, pp. 21-28, 1984.
[11]
S. Mitrus, “Ultrastructure of the calcareous layer eggshell of the turtle Emys orbicularis (L) - preliminary study”. Ann. Acad. Med. Bialostocensis, vol. 42 (suppl. 2), pp. 199-203, 1997.
[12]
J. W. Bickham, J. B. Iverson, J. F. Parham, HD. Philippen, A. G. J. Rhodin, H. B. Shaffer, P. Q. Spinks, P. P. Van Dijk, “An annotated list of modern turtle terminal taxa with comments on areas of taxo-nomic instability and recent change”. Chelonian Res Monogr vol. 4, pp. 173–199, 2007.
[13]
N. Valenzuela, “The Painted Turtle, Chrysemys picta: a model system for vertebrate evolution, ecology, and human health”. Cold Spring Harb Protoc, vol. 4, pp. 1–9, 2009.
[14]
M. A. Ewert, C. R. Etchberger, C. E. Nelson, “Turtle sex determining modes and TSD patterns, and some TSD pattern correlates. In Temperature dependent sex determination in vertebrates”. eds. N Valenzuela and VA Lance, Smithsonian Books, Washington, DC, pp. 21–32, 2004.
[15]
I. Y. Mahmoud, G. L. Hess, J. Klicka, “Normal embryonic stages of western painted turtle, Chrysemys-Picta-Bellii”. J Morphol, vol. 141, pp. 269–279, 1973.
[16]
B. H. Shaffer, P. Minx, D. E. Warren, “The western painted turtle genome, a model for the evolution of extreme physiological adaptations in a slowly evolving lineage”. Genome Biol, R28, 2013.
[17]
D. E. Janes, C. Organ, N. Valenzuela, “New resources inform study of genome size, content and organization in nonavian reptiles”. Integr Comp Biol, vol. 48, pp. 447–453, 2008.
[18]
J. D. Congdon, and J. W. Gibbons, “Turtle eggs: their ecology and evolution. In. Life history and ecology of the slider turtle”. J. W. Gibbons, ed. pp. 109-123. Smithsonian Institution Press, Washington, D C. 1990.
[19]
J. Gibbons, P. Geetha-Loganathan, “Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy”. J. Vis. Exp, vol. 150, 2019.
[20]
G. A Cordero, F. J. Janzen, “An Enhanced Developmental Staging Table for the Pianted Turtle, Chrysemys picta (Testudines: Emydidae)”. Journal of Morphology, vol. 275, pp. 442-455, 2014.
[21]
G. C. Packard, M. J. Packard, “Evolution of the cleidoic egg among reptilian antecedents of birds”. Am. Zool, vol. 20, pp. 351-362, 1980.
[22]
S. E. Solomon, T. Baird, “Studies on the egg shell (oviductal and oviposited) of Cheloniamydas”. L. J. Exp. Mar. Biol. Ecol, vol. 22, pp. 145-160, 1976.
[23]
E. H. Chan, S. E. Solomon. “The structure and function of the eggshell of the leatherback turtle, (Dermochelys coriacea) from Malaysia, with notes on infective fungal forms”. Animal Technology, vol. 40, pp. 91-102, 1989.
[24]
S. Kusuda, Y. Yasukawa, H. Shibata, T. Saito, D. Osamu, Y. Ohya, N. Yoshizaki, “ Diversity in the Matrix Structure of Eggshells in the Testudines (Reptilia). Zoological science, vol 30. pp. 366-74, 2013.
[25]
H. Silyn-Roberts, R. M. Sharp. “Preferred orientation of calcite and aragonite in the reptilian eggshells”. Proc R Soc Lond B, vol. 225, pp. 445-455, 1985.
[26]
H. Silyn-Roberts, R. M Sharp, “Crystal Growth and the Role of the Organic Network in Eggshell Biomineralization”. Proceedings of The Royal Society of London. Series B, Biological Sciences (1934-1990), vol. 227, pp. 303-324, 1986.
[27]
M. J. Packard, T. M. Short, G. C. Packard, and T. A. Gorell, “Sources of calcium for embryonic development in eggs of the snapping turtle Chelydra serpentirw”. J. Exp. Zool, vol. 230, pp. 81-87, 1984b.
[28]
R. Bellairs, and A. Boyde, “Scanning electron microscopy of the shell membranes of the hen's eggs”. Z. Zellforsch, vol. 96, pp. 237-249, 1969.
[29]
H. K. Erben, (1970) “Ultrastrukturen und Mineralisation rezenter und fossiler Eischalen bei Vogeln und Reptilien”. Biomineralisation, vol. 1, pp. 1-66, 1970.
[30]
K. Simkiss, “The structure and formation of the shell and shell membranes. In T. C. Carter (ed): Egg Quality/A Study of the Hen's Egg”. British Egg Marketing Board Symposium Number 4, pp. 3-25, 1968.
[31]
J. D. Young, “The structure and some physical properties of the testudinian eggshell”. Proc. Zool.&C. London, vol. 120, pp. 455-469, 1950.
[32]
K. Mao, M. Murakami, A. Iwasawa, N. Yoshizaki, “The asymmetry of avian egg-shape: An adaptation for reproduction on dry land”. J. Anat, vol. 210, pp. 741–748, 2007.
[33]
N. Yoshizaki, H. Saito, “Changes in shell membranes during the development of quail embryos”. Poult. Sci. vol. 81, pp. 246–251, 2002.
[34]
N. Yoshizaki, M. Soga, Y. Ito, K. M. Mao, S. Fowzia, S. Yonezawa, “Two-step consumption of yolk granules during the development of quail embryos”. Dev. Growth Differ, vol. 46, pp. 229–238, 2004b.
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